Other
Part:BBa_K2996506:Design
Designed by: Shifeng Jiang Group: iGEM19_SJTU-BioX-Shanghai (2019-10-03)
Combination of sgRNA production module and target-DBD binding site
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 287
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 199
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 287
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 287
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We found that part BBa_K2996504 was not a protein coding sequnce.However,another plasmid contains both sgRNA production module and C-luciferase-DBD sequence.That was too much burden for the latter plasmid.We put the sgRNA production module to the former plasmid,to the upstream of BBa_K2996504.
Source
Sythesis