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Part:BBa_K2996506:Design

Designed by: Shifeng Jiang   Group: iGEM19_SJTU-BioX-Shanghai   (2019-10-03)


Combination of sgRNA production module and target-DBD binding site


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 287
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 199
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 287
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 287
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We found that part BBa_K2996504 was not a protein coding sequnce.However,another plasmid contains both sgRNA production module and C-luciferase-DBD sequence.That was too much burden for the latter plasmid.We put the sgRNA production module to the former plasmid,to the upstream of BBa_K2996504.


Source

Sythesis

References